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alix  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc alix
    Alix, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 482 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+alix+antibody/Alix+Mouse+mAb/pmc13035530-39-20-21
    Average 96 stars, based on 482 article reviews
    alix - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    other:

    Article Title: Site-specific adaptive nanovesicles for oral insulin delivery
    Article Snippet: Anti-CD63 antibody (#10112), anti-CD9 antibody (#98327), and anti-Alix antibody (#92880) were purchased from Cell Signaling Technology.

    Article Title: Site-specific adaptive nanovesicles for oral insulin delivery.
    Article Snippet: Anti- CD63 antibody (#10112), anti- CD9 antibody (#98327), and anti- Alix antibody (#92880) were purchased from Cell Signaling Technology.

    Western Blot:

    Article Title: Humoral regulation of iron metabolism by extracellular vesicles drives antibacterial response.
    Article Snippet: GR3417018-5, dilution1:1000 for WB); anti-Salmonella typhimurium LPS antibody (Mouse monoclonal 1E6, Abcam, ab8274, lot.no. .. GR3398476-1, dilution: 1:200 for IF); anti-FPN1 antibody (Rabbit polyclonal, Invitrogen, PA5-115915, dilution 1:1000 for WB); antiCD63 antibody (mouse monoclonal MX-49.129.5, Santa Cruz Biotechnology, cat. no. sc-5275, lot. no. H2418, dilution: 1:1000 for WB); anti-FTH1 antibody (Cat#3998S, Cell Signaling Technology, Lot.no.2, dilution: 1:1000 for WB); anti-Alix antibody (Cat#92880, Cell Signaling Technology, Lot.no.1, dilution: 1:1000 for WB); anti-ATF4 antibody (Cat#11815, Cell Signaling Technology, Lot.no.2, dilution: 1:1000 for WB); anti-IRE1 antibody (Cat#3294P, Cell Signaling Technology, Lot.no.12, dilution: 1:1000 for WB); anti-TFEB antibody (Cat#32361S, Cell Signaling Technology, Lot.no.2, dilution: 1:1000 for WB); anti-Erk1/2 antibody (Cat#9102s, Cell Signaling Technology, Lot.no.27, dilution: 1:1000 for WB); anti-Phospho-Erk1/2 antibody (Cat#9101s, Cell Signaling Technology, Lot.no.30, dilution: 1:1000 for WB); anti-F4/80 antibody (Rat monoclonal CI:A3-1, Abcam, ab6640, lot. no. GR3250648-1, dilution: 1:200 for IF); 3 nature portfolio | rep orting sum m ary M arch 2021 Validation Animals and other organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research Laboratory animals Wild animals Field-collected samples anti-Macrophage antibody (Mouse monoclonal MAC387, Abcam, ab22506, lot. no. 864160, dilution: 1:200 for IF); anti-GAPDH antibody (YESEN, Cat.30201ES60, Lot.no.G3112040, dilution: 1:1000 for WB); peroxidase AffiniPure goat anti-mouse IgG (DIYIBio, Cat.no. .. DY60203); peroxidase AffiniPure goat anti-rabbit IgG (DIYIBio, Cat.no.

    Article Title: Two-step evolution of HIV-1 budding system leading to pandemic in the human population.
    Article Snippet: At 48 h after lentiviral transduction, the cells were expanded and selected with 1 ng/mL Blasticidin (Invivogen, cat# ant-bl-1) and 0.5 ng/mL Puromycin (Invivogen, cat# ant-pr-1). .. Finally, the deletion of the endogenous PDCD6IP gene (encoding ALIX) was verified by Western blotting using an anti-ALIX antibody (Cell signaling, Cat# 2171S) (Figure S3B). .. Finally, the deletion of the endogenous PDCD6IP gene (encoding ALIX) was verified by Western blotting using an anti-ALIX antibody (Cell signaling, Cat# 2171S) (Figure S3B).

    Biomarker Discovery:

    Article Title: Humoral regulation of iron metabolism by extracellular vesicles drives antibacterial response.
    Article Snippet: GR3417018-5, dilution1:1000 for WB); anti-Salmonella typhimurium LPS antibody (Mouse monoclonal 1E6, Abcam, ab8274, lot.no. .. GR3398476-1, dilution: 1:200 for IF); anti-FPN1 antibody (Rabbit polyclonal, Invitrogen, PA5-115915, dilution 1:1000 for WB); antiCD63 antibody (mouse monoclonal MX-49.129.5, Santa Cruz Biotechnology, cat. no. sc-5275, lot. no. H2418, dilution: 1:1000 for WB); anti-FTH1 antibody (Cat#3998S, Cell Signaling Technology, Lot.no.2, dilution: 1:1000 for WB); anti-Alix antibody (Cat#92880, Cell Signaling Technology, Lot.no.1, dilution: 1:1000 for WB); anti-ATF4 antibody (Cat#11815, Cell Signaling Technology, Lot.no.2, dilution: 1:1000 for WB); anti-IRE1 antibody (Cat#3294P, Cell Signaling Technology, Lot.no.12, dilution: 1:1000 for WB); anti-TFEB antibody (Cat#32361S, Cell Signaling Technology, Lot.no.2, dilution: 1:1000 for WB); anti-Erk1/2 antibody (Cat#9102s, Cell Signaling Technology, Lot.no.27, dilution: 1:1000 for WB); anti-Phospho-Erk1/2 antibody (Cat#9101s, Cell Signaling Technology, Lot.no.30, dilution: 1:1000 for WB); anti-F4/80 antibody (Rat monoclonal CI:A3-1, Abcam, ab6640, lot. no. GR3250648-1, dilution: 1:200 for IF); 3 nature portfolio | rep orting sum m ary M arch 2021 Validation Animals and other organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research Laboratory animals Wild animals Field-collected samples anti-Macrophage antibody (Mouse monoclonal MAC387, Abcam, ab22506, lot. no. 864160, dilution: 1:200 for IF); anti-GAPDH antibody (YESEN, Cat.30201ES60, Lot.no.G3112040, dilution: 1:1000 for WB); peroxidase AffiniPure goat anti-mouse IgG (DIYIBio, Cat.no. .. DY60203); peroxidase AffiniPure goat anti-rabbit IgG (DIYIBio, Cat.no.



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    Characterization and imaging of serum‐derived cEVs. (A) cEVs images as acquired by transmission electron microscopy; bar represents 100 nm. (B) Concentration and size distribution of cEVs examined by NTA using Nanosight‐NS300. (C) ApoA1 concentration in serum and cEVs. (D) Immunoblot detection of EV protein markers and non‐associated proteins. The parametric Student's t ‐test was used for statistical analysis of ApoA1; *** p < 0.001. <t>ALIX,</t> ALG‐2‐interacting protein X; ApoA1, apolipoprotein <t>A1;</t> <t>CD63,</t> cluster of differentiation 63; cEVs, circulating extracellular vesicles; CTR, controls; nm, nanometer; NTA, nanoparticle tracking analysis; SCD, subjective cognitive decline; TSG101, tumor susceptibility gene 101.
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    Characterization and imaging of serum‐derived cEVs. (A) cEVs images as acquired by transmission electron microscopy; bar represents 100 nm. (B) Concentration and size distribution of cEVs examined by NTA using Nanosight‐NS300. (C) ApoA1 concentration in serum and cEVs. (D) Immunoblot detection of EV protein markers and non‐associated proteins. The parametric Student's t ‐test was used for statistical analysis of ApoA1; *** p < 0.001. <t>ALIX,</t> ALG‐2‐interacting protein X; ApoA1, apolipoprotein <t>A1;</t> <t>CD63,</t> cluster of differentiation 63; cEVs, circulating extracellular vesicles; CTR, controls; nm, nanometer; NTA, nanoparticle tracking analysis; SCD, subjective cognitive decline; TSG101, tumor susceptibility gene 101.
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    alix  (Cell Signaling Technology Inc)
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    Characterization and imaging of serum‐derived cEVs. (A) cEVs images as acquired by transmission electron microscopy; bar represents 100 nm. (B) Concentration and size distribution of cEVs examined by NTA using Nanosight‐NS300. (C) ApoA1 concentration in serum and cEVs. (D) Immunoblot detection of EV protein markers and non‐associated proteins. The parametric Student's t ‐test was used for statistical analysis of ApoA1; *** p < 0.001. <t>ALIX,</t> ALG‐2‐interacting protein X; ApoA1, apolipoprotein <t>A1;</t> <t>CD63,</t> cluster of differentiation 63; cEVs, circulating extracellular vesicles; CTR, controls; nm, nanometer; NTA, nanoparticle tracking analysis; SCD, subjective cognitive decline; TSG101, tumor susceptibility gene 101.
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    Image Search Results


    Characterization and imaging of serum‐derived cEVs. (A) cEVs images as acquired by transmission electron microscopy; bar represents 100 nm. (B) Concentration and size distribution of cEVs examined by NTA using Nanosight‐NS300. (C) ApoA1 concentration in serum and cEVs. (D) Immunoblot detection of EV protein markers and non‐associated proteins. The parametric Student's t ‐test was used for statistical analysis of ApoA1; *** p < 0.001. ALIX, ALG‐2‐interacting protein X; ApoA1, apolipoprotein A1; CD63, cluster of differentiation 63; cEVs, circulating extracellular vesicles; CTR, controls; nm, nanometer; NTA, nanoparticle tracking analysis; SCD, subjective cognitive decline; TSG101, tumor susceptibility gene 101.

    Journal: Alzheimer's & Dementia : Translational Research & Clinical Interventions

    Article Title: A potential multimodal biomarker – cognitive signature associated with the conversion from subjective cognitive decline to mild cognitive impairment

    doi: 10.1002/trc2.70240

    Figure Lengend Snippet: Characterization and imaging of serum‐derived cEVs. (A) cEVs images as acquired by transmission electron microscopy; bar represents 100 nm. (B) Concentration and size distribution of cEVs examined by NTA using Nanosight‐NS300. (C) ApoA1 concentration in serum and cEVs. (D) Immunoblot detection of EV protein markers and non‐associated proteins. The parametric Student's t ‐test was used for statistical analysis of ApoA1; *** p < 0.001. ALIX, ALG‐2‐interacting protein X; ApoA1, apolipoprotein A1; CD63, cluster of differentiation 63; cEVs, circulating extracellular vesicles; CTR, controls; nm, nanometer; NTA, nanoparticle tracking analysis; SCD, subjective cognitive decline; TSG101, tumor susceptibility gene 101.

    Article Snippet: Proteins were incubated for 30 min with primary antibodies against TSG101 (Novus Biologicals LLC, Catalog No.: NB200–112, 1:10), CD63 (R&D Systems; Catalog No.: MAB50482, 1:20), ALIX (R&D Systems; Catalog No.: MAB50482, 1:20), and calnexin (Novus Biologicals LLC; Catalog No.: NB100–1965, 1:25), followed by washing and incubation with species‐specific secondary antibodies.

    Techniques: Imaging, Derivative Assay, Transmission Assay, Electron Microscopy, Concentration Assay, Western Blot